The role of functional diversity in forest restoration planting
The Functional Diversity Experiment is a large-scale tropical forest restoration study designed to test how species selection based on functional traits influences early forest recovery in highly degraded landscapes. While assisted forest restoration is often necessary where natural regeneration is slow or unlikely, it is also resource-intensive, making efficient species selection critical. Rather than prioritizing rapid growth or species richness alone, this experiment applies a functional diversity framework to restoration planting design, explicitly testing whether species assemblages with diverse ecological strategies can accelerate the recovery of forest structure and ecosystem functioning.
The experiment was established beginning in 2021 across four degraded tropical wet forest sites in southwestern Costa Rica, spanning a range of tropical forest types. Experimental plots were planted along a gradient of functional diversity, ranging from monocultures to highly functionally diverse mixtures of up to twelve native tree species. Species were selected based on key functional traits related to growth strategy, resource acquisition, and life history, allowing the experiment to disentangle the effects of functional diversity from species number alone. This design enables comparisons across a range of functional diversity in restoration tree planting. At each site, six treatments are replicated six times, resulting in 36 plots measuring 15 x 15 m and containing 48 trees per plot. Each site occupies approximately 3 hectares of degraded pasture.
In collaboration with researchers at ETH Zürich, OTS, Asociación Ambiental Finca Cántaros, La Gamba Tropical Station, Loma Linda Field Station, and Finca Aguas Buenas, we are evaluating how restoration plantings designed around functional diversity influence early forest recovery. We are measuring changes in vegetation structure, seed rain, soil and litter organism biodiversity, bird communities, and soil nutrient dynamics. Together, these sites allow us to assess how functional diversity shapes forest recovery across varied ecological conditions and at a broader regional scale. Ultimately, the Functional Diversity Experiment seeks to bridge restoration ecology theory and on-the-ground practice by offering practical guidance for designing resilient, functionally rich tropical forests under real-world restoration constraints.
More information on the experimental design and preliminary results is available in this video (https://drive.google.com/file/d/1OKGPWuhzPQEef0Qg428RMZw_jPIEo6XY/view?usp=drive_link).
NATURAL REGENERATION VS. TREE PLANTING DISTRIBUTED EXPERIMENT
Background:
Many studies have highlighted the tremendous utility of using passive restoration (i.e., natural regeneration) to restore tropical forests in a cost-effective manner (Crouzeilles et al., 2017; Poorter et al., 2016). Still others demonstrate that active tree planting can more effectively accumulate carbon than natural regeneration (Philipson et al., 2020; Wheeler et al., 2016). Additionally, passive and active restoration outcomes are difficult to directly compare because passive restoration is generally studied in less disturbed sites that do not require active interventions (e.g., tree planting; Reid et al., 2018). To move past the site-selection bias inherent in many studies comparing passive vs. active restoration, we are seeking sites across Costa Rica to join a distributed restoration experiment. Our ultimate goal is to compare how rates of forest recovery vary across the two most common interventions applied in tropical forests: natural regeneration (passive restoration) vs. tree planting (active restoration).
Experimental goals:
Our initial goals are to compare recovery rates of vegetation structure and carbon sequestration, plant species richness and functional diversity, and soil carbon and fertility vary between natural regeneration vs. tree planting restoration interventions. If time allows we will examine how other aspects of the ecosystem recover as well (e.g., vertebrate and invertebrate community, soil microbiome).
Experimental implementation:
At sites where your organization is already carrying out restoration, the aim to pair “natural regeneration” (NR) with “tree planting” (TP) treatment plots so that the outcomes can be compared.
To the extent possible, the areas under each pair of treatments should be a similar as possible (i.e., same soil types, slope, similar existing vegetation or grass type).
We will provide guidance on how to sample initial vegetation and on how to monitor growth of planted and naturally regenerating woody vegetation. The first monitoring should be done immediately after the tree planting and then every two years.
For obvious reasons, it is important to leave the naturally
regeneration treatments unplanted and protected in the same
way as the tree planting treatments.
Requirements to become a site:
An established program using active tree planting of native and/or naturalized species for forest restoration. The tree planting approach must be targeted to restore native forest, and can include plantation style planting, spatially patterned planting (e.g., strips, clusters) or riparian strip restoration planting.
Areas that are mostly cleared of existing vegetation and/or have not been regenerating for more than three years.
Space to install a minimum of six plots (min. size: 0.10 hectare; ~1000 m2 or ~32 x 32 m) per intervention (natural regeneration vs. tree planting).
A person on staff who is responsible for overseeing the
collaborative project and who will be responsive to
communication and facilitate data collection.
What we offer:
Participation in a large-scale collaborative project over a 5+ year timeframe
Training for staff on monitoring methods, technical support and possible on-site support
Consulting and guidance for project establishment and initial data collection
Co-authorship on collaborative papers
Opportunity to lead publications based on supplementary data sets collected at your site
Networking opportunities and visibility
Participation in workshops / training on new technologies
for restoration monitoring
What we ask:
Partner organizations must comply with all local laws, including any referring to international agreements
Project protocols must be followed carefully, and sites must be sampled according to the project schedule (every two years)
Sign a data sharing agreement and upload/share data
Partners are responsive to communications and questions related to project
Co-authors must comment on the respective manuscripts and/or indicate that they agree with the content of the work
Partners must share project-related photos & video for
outreach and communications purposes
Quantifying recovery rates:
We will provide a variety of protocols to track: (1) rates
of biomass accumulation and (2) rates of biodiversity
recovery
General Timeline:
Experiment installed: By end of 2027 * We can also work with
already established planted areas assuming that there are
similar, nearby unplanted areas with similar
characteristics.
Baseline data collection commences: Immediately after active
restoration (tree planting) is completed.
Bi-annual survey of recovery indicators: Every two years
during growing season
A small river named Duden flows by their place and supplies it with the necessary regelialia.
A small river named Duden flows by their place and supplies it with the necessary regelialia.
A small river named Duden flows by their place and supplies it with the necessary regelialia.